This isn't a dyno tuning problem. After installing the SE Stage 2 cams and big TBs the bike had the flat spot from hell at 3500 rpm and the hp curve went down from 4000-5000 rpm. I took it to a dyno shop that tunes a lot of V-Rods and after a couple of hours the tuner had the bike running beautifully. He spent a great deal of time working on the 3500-5000 rpm range and the 7500-9000 rpm range. That is why I went topless, to cure the roll off in power after 7500 rpm we had with the full air box in place (even with my oversized intake snorkel). When he was finished the bike idled and ran smoother than it ever did. It was so smooth it was eerie.i had a vrod in here with the same symptoms you describe and the dyno tec couldnt get rid of the stutter upon roll on.i tested the tps and it seemed fine ,but replaced it anyway.fixed the problem.they are cheep and its good to eliminate that as the problem.
This problem is something different. At cold start it only fires on one cylinder and the problem did not show up until a week or so ago. Prior to that there was no problem. I either developed a pinhole leak in a fuel hose, the fuel pressure regulator has a problem or the fuel system has somehow been contaminated. When we check the pressure Thursday afternoon I will know more.